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| 产品名称： | c9332 |
|---|---|
| 商品货号： | TS165300 |
| Designations： | c9332 |
| GenBank Number： | U00060 U10556 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Insert： | DNA: genomic Insert lengths(kb): 40.52799987792969 Gene product: CDC12 |
| Insert Size (kb)： | 40.528 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--7.0, 3.5 (doublet), 2.85 (doublet), 2.55 (doublet), 2.4, 1.55 (triplet), 1.2 (doublet) and smaller bands. Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.724, 3.505, 3.505, 2.922, 2.899, 2.772 (vector), 2.547, 2.547, 2.387, 1.630, 1.569, 1.569, 1.238, 1.222, 0.827, 0.777, 0.697, 0.642, 0.494, 0.430, 0.429 (vector). IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 270879-311407 of the sequence of chromosome VIII. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Gimeno CJ, Fink GR. Induction of pseudohyphal growth by over expression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. Mol. Cell. Biol. 14: 2100-2112, 1994. PubMed: 8114741 Linda Riles, personal communication |