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| 产品名称： | CHAROMID 9-20 |
|---|---|
| 商品货号： | TS165308 |
| Designations： | CHAROMID 9-20 |
| Depositors： | I Saito |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 20.0000000000000000 Vector: CHAROMID 9-20 (cosmid) Construction: pTBE-PL9 and multiple copies of a 2 kb AvaI spacer Marker(s):ampR Construct size (kb): 20.0 Features: marker(s): ampR replicon: pMB1 terminator: none enhancer: none |
| Applications： | Host should be mk+ because some packaging mixes destroy charomids by EcoK. Useful for cloning 18-32 kb fragments. |
| Comments： | Dimer vector can be packaged, so cosmid-type arm preparation or phosphatase treatment is necessary. Host should be mk+ because some packaging mixes destroy charomids by EcoK. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Useful for cloning 18-32 kb fragments. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Saito I, Stark GR. Charomids: Cosmid vectors for efficient cloning and mapping of large or small restriction fragments. Proc. Natl. Acad. Sci. USA 83: 8664-8668, 1986. PubMed: 3022301 |
| Shipped： | freeze-dried |