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| 产品名称： | pAqpZ plasmid in E. coli |
|---|---|
| 商品货号： | TS165338 |
| Designations： | pAqpZ plasmid in E. coli |
| GenBank Number： | U38664 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | P Agre |
| Applications： | ecause of the XhoI and SalI sites in the MCS 5 to the insert, either enzyme can be used to excise an intact open reading frame. |
| Vector： | Construct size (kb): 3.774 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.813 Gene product: aquaporin Z aqpZ* |
| Insert Size (kb)： | 0.813 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/NotI--2.9, 0.84; SalI--2.9, 0.84; XhoI--2.9, 0.84. The insert contains the following restriciton sites (approximate kb from the 5end): SnaBI--0.25; BsmI--0.39; PacI--0.69. The insert in this clone contains the complete open reading frame for the E. coli aquaporin Z aqpZ* gene plus 100 bp of the 3 untranslated region.After the 3 UTR, a SalI and XhoI restriction site have been added.Because of the XhoI and SalI sites in the MCS 5 to the insert, either enzyme can be used to excise an intact open reading frame. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Calamita G, et al. Molecular cloning and characterization of AqpZ, a water channel from Escherichia coli. J. Biol. Chem. 270: 29063-29066, 1995. PubMed: 7493926 |