微信小陈
微信小章
产品简介
购买须知
| 产品名称： | Human Coxsackievirus A6 |
|---|---|
| 商品货号： | TS165368 |
| Classification： | Picornaviridae, Enterovirus, Human enterovirus A |
| Agent： | Human Coxsackievirus A6 |
| Strain： | Gdula |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C |
| Images： |  |
| Comments： | Derived by adaptation of primary cell culture product ATCC® VR-165™ to RD cells (ATCC® CCL-136™).xa0 ATCC® VR-165™ and ATCC® VR-1801 have not been compared for sequence of infectivity in primary cell culture and tissue culture. |
| Effect on Host： | CPE, cell rounding and death |
| Recommended Host： | RD cells (ATCC® CCL-136™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer with DPBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | HA Wenner |
| Source： | Stool from 5-year-old child with sore throat, fever and leg muscle spasm, New York, 1949. |
| References： | Dalldorf G. The coxsackie virus group. Ann. N.Y. Acad. Sci. 56: 583-586, 1953. PubMed: 13139263 |