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微信小章| 产品名称： | U-CH1 |
|---|---|
| 商品货号： | TS165519 |
| Organism： | Homo sapiens, human |
| Tissue： | sacral bone |
| Product Format： | frozen |
| Morphology： | mesenchymal like, with variable vacuoles |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | tumor, chordoma |
| Age： | 56 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | Use as a model of chordoma which is a rare slow-growing tumor
The Chordoma Foundation may be able to offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information. |
| Shipping Information： | frozen |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | This cell line was established from a local recurrence of a sacrococcygeal after radiotherapy 4 years after initial surgery. |
| Clinical Data： | 56 years male Caucasian |
| Genes Expressed： | overexpressionxa0of transcription factor T (brachyury) |
| Comments： | U-CH1 is the first human chordoma cell line. It exhibits chordoma-like characteristics and has molecular, genetic, and morphological features typical of chordoma. This cell line was established from a local recurrence of a sacrococcygeal chordoma after radiotherapy 4 years after initial surgery. Chordoma is a rare slow-growing tumor type, and U-CH1 is a relatively slow-growing cell line. U-CH1 has a heterogeneous morphology consisting of physaliferous cells with mcinous intercellular substance, which represent typical chordoma features. The cells overexpressxa0the transcription factor T (Brachyury) that is the most specific marker for chordoma. This cell line was accessioned with the support of the Chordoma Foundation, a non-profit organization working to improve the lives of chordoma patients by accelerating research to develop effective treatments for the chordoma disease. |
| Complete Growth Medium： | Iscoves Modified Dulbeccos Medium (IMDM; ATCC® No. 30-2005): RPMI-1640 Medium (ATCC® No. 30-2001) (4:1) + 10% FBS (ATCC® No. 30-2020) + additional 1% L-glutamine (ATCC® No. 30-2214) |
| Subculturing： | Coating description: Dilute rat tail type I collagen (BD Biosciences, Catalog No. 354236) to 50 μg/ml. Add 7.5 ml coating buffer to flask and incubate at room temperature for one hour. Carefully aspirate remaining solution. Rinse flask 2 times to remove acid, using 1x DPBS. Coated flasks may be used immediately or stored at 2-8°C up to one week under sterile conditions. Volumes used in this subculture protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
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| Cryopreservation： | Freeze medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO |
| Culture Conditions： | Temperature: 37oC Atmosphere: air, 95%; carbon dioxide (CO2),xa05% |
| STR Profile： | xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0TH01: 7 |
| Passage Number： | 30 |
| Name of Depositor： | Silke Bruderlein, Peter Moller |
| Deposited As： | Homo sapiens |
| Year of Origin： | August 17, 1998 |
| References： | Scheil S, et al. Genome-wide analysis of sixteen chordomas by comparative genomic hybridization and cytogenetics of the first human chordoma cell line, U-CH1. Genes Chromosomes Cancer 32(3): 203-211, 2001. PubMed: 11579460 Bruderlein S, et al. Molecular characterization of putative chordoma cell lines. Sarcoma 2010: 630129. Epub 2010 Dec 30. PubMed: 21253487 Presneau N, et al. Role of the transcription factor T (brechyury) in the pathogenesis of sporadic chordoma: a genetic and functional-based study. J. Pathol. 2239(3): 327-335, 2011. PubMed: 21171078 Liu X, et al. Establishment and Characterization of a Novel Chordoma Cell Line: CH22. J. Orthip Res. 30 (10): 1666-1673, 2010. PubMed: 22504929 |