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| 产品名称： | TeloHAEC |
|---|---|
| 商品货号： | TS165714 |
| Organism： | Homo sapiens, human |
| Tissue： | aorta |
| Cell Type： | endothelial |
| Product Format： | frozen |
| Morphology： | endothelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells containing SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 34 years old |
| Gender： | female |
| Applications： | TeloHAEC (TS165714) cells retain important endothelial cell characteristics such as xa0CD31/PECAM-1 marker expression and LDL functional uptake; the cells also show effective inflammatory response upon TNFα treatment and increase proliferation upon VEGF stimulation. xa0When co-cultured with fibroblasts, TeloHAEC cells can also form neoangiogenic tubular networks in vitro, which are responsive to VEGF stimulation and suramin inhibition. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a diploid cell line of female origin with a consistent normal kayrotype at low and high passages |
| Images： | 
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| Derivation： | TeloHAEC is a clonal cell line immortalized by stably expressing human telomerase catalytic subunit hTERT |
| Clinical Data： | femalexa0
34 |
| Antigen Expression： | Positive for CD31/PECAM-1 expression xa0and capable of uptaking Low Density Lipoprotein (LDL). |
| Complete Growth Medium： | The base medium for this cell line is Vascular Cell Basal Medium (ATCC PCS-100-030), supplemented with Vascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041). xa0 Optional: Add 0.3ug/mL Puromycin(Santa Cruz Biotech: sc-108071A). Note: Do not filter complete medium. |
| Subculturing： | Volumes used in this protocol are for 75 cm2xa0flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes. Subculture when the culture is about 90% confluent.
|
| Cryopreservation： | 90% FBS, 10% DMSO |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | D13S317: 9, 12 D7S820: 10, 11 xa0 xa0 xa0 xa0 D16S539: 12, 13 vWA: 15, 16 xa0 xa0 xa0 xa0 xa0 xa0xa0 Amelogenin: xa0X TPOX: 8 CSF1PO: 11, 12 TH01: 6, 8 |
| Population Doubling Level (PDL)： | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor： | ATCC |