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| 产品名称： | pBM2240 |
|---|---|
| 商品货号： | TS166038 |
| Designations： | pBM2240 |
| Depositors： | M Johnston |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.2500000000000000 Vector: pBM2240 (phagemid) Construction: pRS316, lambdaMG3 Marker(s):URA3,ampR Construct size (kb): 7.25 Features: marker(s): ampR, URA3 replicon: pMB1, CEN6/ARS4 centromere: CEN6 |
| Applications： | shuttle vector vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--7.3; KpnI--5.6, 1.5; BamHI/XbaI--6.4, 0.9. S. cerevisiae is cotransformed with the lambda clone of interest and vector linearized with an EcoRI/XhoI double digest. Ura+ colonies are tested for complementation of a mutant phenotype. YC-type shuttle vector designed to clone yeast genes that are difficult to clone by rescuing inserts from the vector lambdaMG3 by homologous recombination. Constructed by insertion of a 0.858 kb BamHI-XbaI fragment and a 1.5 kb KpnI fragment from lambdaMG3 into the corresponding sites of pRS316 (ATCC 77145). The order of the major features in this plasmid is: f1 ori - lacZ - T7 promoter - KpnI/lambdaMG3/KpnI - MCS - BamHI/lambdaMG3/XbaI - T3 promoter - pMB1 ori - ampR - CEN6/ARS4 - URA3. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Erickson JR, Johnston M. Direct cloning of yeast genes from an ordered set of lambda clones in Saccharomyces cerevisiae by recombination in vivo. Genetics 134: 151-157, 1993. PubMed: 8514124 |
| Shipped： | freeze-dried |