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| 产品名称： | pKB663 |
|---|---|
| 商品货号： | TS166064 |
| Designations： | pKB663 |
| GenBank Number： | M10431 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | OmniGene, Inc., KC Backman, OmniGene, Inc. |
| Applications： | produces protein chorismate mutase-P-prephenate dehydrogenase |
| Vector： | Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pKB430 Intact vector size: 4.500 Type of vector: plasmid Vector end: PvuII Vector end: BamHI Cloning sites: PvuII Polylinker sites: Other unique sites: BamHI Construction: pBR322, lac promoter Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, -> replicon: pMB1 promoter for expression: lac, -> restriction site: PvuII marker(s): tetR, Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Escherichia coli Gene symbol: pheA Gene name: chorismate mutase-P-prephenate dehydrogenase Contains complete coding sequence?: Y Type of DNA: genomic Insert 5 end: StuI Insert 3 end: BglII Insert size (kb): 2.17 Cross references: DNA Seq. Acc.: M10431 Nucleotides 1-2168 of the insert correspond to nucleotides 576-2743 of M10431. Insert lengths(kb): 2.170000076293945 Gene product: chorismate mutase-P-prephenate dehydrogenase pheA Target Gene: chorismate mutase-P-prephenate dehydrogenase |
| Insert Size (kb)： | 2.170 |
| Media： | ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; EcoRI/BamHI--4.0, 1.08; EcoRI/NcoI--3.5, 1.5. Excision of the insert with EcoRI+Tth111I yields a lac promoter - pheA cassette. The construct contains a weak attenuator sequence at the 5 end of the pheA coding sequence. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Backman KC. Method of biosynthesis and cells therefor. US Patent 4,839,286 dated Jun 13 1989 Backman KC. Enzyme deregulation. US Patent 4,753,883 dated Jun 28 1988 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |