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| 产品名称： | hARa CL7a-AR 160-910 |
|---|---|
| 商品货号： | TS166110 |
| Designations： | hARa CL7a-AR 160-910 |
| GenBank Number： | M34233 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein androgen receptor (dihydrotestosterone receptor; testicular feminization) |
| Vector： | Construct size (kb): 8.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pKCR-2 Intact vector size: 5.700 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI, EcoRI Polylinker sites: Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: AR Other gene symbols: DHTR Genomic copy number: unique Gene name: androgen receptor (dihydrotestosterone receptor; testicular feminization) Contains complete coding sequence?: N Chromosome: X; Localization: X q11.2-q12 Tissue: testis Type of DNA: cDNA Insert end: Modification: BamHI linkers Insert end: Modification: BamHI linkers Insert size (kb): 2.8 Cross references: DNA Seq. Acc.: M34233 Insert lengths(kb): 2.799999952316284 Tissue: testis Gene product: androgen receptor (dihydrotestosterone receptor; testicular feminization) AR Target Gene: androgen receptor (dihydrotestosterone receptor; testicular feminization) |
| Insert Size (kb)： | 2.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.0, 2.7; HindIII--8.7; KpnI--7.3, 1.35; EcoRI--4.9, 2.0, 0.64 (doublet), 0.5; PstI--4.4, 1.8, 1.7, 0.54. Digestion with EcoRI yields two fragments of 2.0 and 0.5 kb. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.2, 0.4, 0.7, 0.75; EcoRI--1.9; HindIII--1.2; KpnI--1.0; NcoI--0.3, 1.75; PstI--0.5; PvuI--2.0; PvuII--0.05, 0.6; SacI--0.5, 1.2. Encodes aa 160-910, including the DNA-binding domain and the hormone-binding domain. Also encodes 16 Gly between aa 447 and 464. Cross hybridizes with a 6.8 kb rat liver mRNA. pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals. |
| References： | Govindan MV. Specific region in hormone binding domain is essential for hormone binding and trans-activation by human androgen receptor. Mol. Endocrinol. 4: 417-427, 1990. PubMed: 2342476 |