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| 产品名称： | lambdafoo |
|---|---|
| 商品货号： | TS166236 |
| Designations： | lambdafoo |
| Depositors： | IN Maruyama |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 43.0000000000000000 DESCRIPTION OF VECTOR: Intact vector size: 43.000 Type of vector: phage Cloning sites: HindIII BamHI SacI EcoRI Polylinker sites: HindIII BamHI SacI EcoRI Construction: lambda2001, lambda gene V Host range: Escherichia coli Features (with orientation and position when available): coding sequence: gene V N-terminal 176 aa, -> other: amber stop codon, -> restriction site: SfiI other: Pro-Thr box encoding a linker peptide, -> insert detection: lacZ, -> ribosome-binding site: Shine-Dalgarno, -> initiation codon: ATG, -> MCS: HindIII...EcoRI, -> Vector: lambdafoo (phage, lambda - replacement) Construction: lambda2001, lambda gene V Construct size (kb): 43.0 Features: initiation codon: ATG insert detection: lacZ other: Pro-Thr box encoding a linker peptide other: amber stop codon MCS: HindIII...EcoRI restriction site: SfiI ribosome-binding site: Shine-Dalgarno coding sequence: gene V N-terminal 176 aa |
| Applications： | expression vector vector for constructing cDNA libraries vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--33.0, 9.4; EcoRI--33.0, 9.4; BglII--22.0, 8.8, 4.8, 4.6, 3.1; PstI--9.6, 9.0, 4.6, 3.2, 2.9, 2.8, 2.4, 2.2, 1.9, 1.9, 1.5. Vector allowing expression of cloned inserts as a fusion protein on the phage particle surface. Inserts are fused to the C-terminus of a truncated phage tail protein by a peptide linker. Presence of the lacZalpha coding sequence and ribosome binding site allow blue-white color detection of recombinants, as well as allowing expression of a cloned insert separate from the phage tail protein. The Pro-Thr box encodes alternating prolyl and threonyl residues, which form a link between the N-terminal phage tail protein and the foreign protein. The linker resembles the hinge-region of IgA1 and allows separation of the foreign protein from the phage particles by digestion with enzymes such as Cellulomonas fimi protease or collagenase. Production of large amounts of fusion protein may inhibit phage assembly. A host allowing low efficiency suppression of the amber mutation is recommended. |
| Media： | ATCC® Medium 1592: SM buffer |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Maruyama IN, et al. Lambda foo: a lambda phage vector for the expression of foreign proteins. Proc. Natl. Acad. Sci. USA 91: 8273-8277, 1994. PubMed: 8058794 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |