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| 产品名称： | pGMC |
|---|---|
| 商品货号： | TS166350 |
| Designations： | pGMC |
| GenBank Number： | X52259 |
| Species： | Homo sapiens, human |
| Depositors： | ML Lane |
| Vector： | Construct size (kb): 3.160000085830689 |
| Insert： | DNA: genomic Insert lengths(kb): 0.4199999868869782 Tissue: DNA from NIH 3T3 cells transformed by MCF7 DNA Gene product: sec oncogene SEC* |
| Insert Size (kb)： | 0.420 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HincII/AccI--uncut; PvuII--2.35, 0.8; HindIII--3.1; EcoRI--3.1; EcoRI/HindIII--2.6, 0.5. The insert extends from the PvuII site at position 41 of the published sequence to the AccI site at 462. Coding sequences extend from 21 to 347. The insert is oriented such that transcription from the SP6 promoter gives the sense strand and from the T7 promoter gives the antisense strand. |
| References： | Lane MA, et al. Nucleotide sequence of a human oncogene active in tumors of secretory epithelium. Nucleic Acids Res. 18: 3068, 1990. PubMed: 2349114 Lane MA, et al. Activation of related transforming genes in mouse and human mammary carcinomas. Proc. Natl. Acad. Sci. USA 78: 5185-5189, 1981. PubMed: 6272293 Mary Ann L Lane, personal communication |