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| 产品名称： | Human respiratory syncytial virus |
|---|---|
| 商品货号： | TS166367 |
| Classification： | Paramyxoviridae, Pneumovirus |
| Agent： | Human respiratory syncytial virus |
| Strain： | ATCC-2012-11 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | This item was isolated in cell culture by three passages in HEp-2 cells This strain belongs to subtype B |
| Effect on Host： | CPE, refractile cell rounding and syncytia |
| Recommended Host： | HEp-2 (ATCC® CCL-23™) |
| Growth Conditions： | Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.1-0.5 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | ATCC |
| Source： | Specimen from a clinical patient in Virginia, 2012 |