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| 产品名称： | delta p53 |
|---|---|
| 商品货号： | TS166439 |
| Designations： | delta p53 |
| GenBank Number： | X02469 |
| Species： | Homo sapiens, human |
| Depositors： | J Kim, R McLinden |
| Applications： | Expression from this construct has been used to quantitate p53 expression levels. The following primers can be used for RT-PCR to detect transcription of the cloned insert: Forward 5 - CATCTACAAGCAGTCACA - 3; Reverse 5 - GCAGTGCTCGCTTAGTGC - 3. The amplified product size is 442 bp. The following oligonucleotide sequence can be used as an internal probe to detect the amplified product: 5 - ATCCACTACAACTACATG - 3. |
| Vector： | Construct size (kb): 3.299999952316284 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-4Z Intact vector size: 3.000 Type of vector: plasmid Vector end: HincII Vector end: HincII Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: TP53 Genomic copy number: unique Gene name: tumor protein p53 (Li-Fraumeni syndrome) Contains complete coding sequence?: N Chromosome: 17; Localization: 17 p13.1 Tissue: SV40-transformed fibroblast Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.6 Cross references: DNA Seq. Acc.: X02469 Nucleotides 1-567 of the insert correspond to nucleotides 600-1167 of X02469. Insert lengths(kb): 0.6000000238418579 Tissue: SV40-transformed fibroblast Gene product: tumor protein p53 (Li-Fraumeni syndrome)( tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen) TP53 Alleles: D2, C2, C1, A1, A1, B2, B1, D1, A2, A2 |
| Insert Size (kb)： | 0.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--2.7, 0.6; EcoRI--3.3; BamHI--3.3. Expression from this construct has been used to quantitate p53 expression levels. The following primers can be used for RT-PCR to detect transcription of the cloned insert: Forward 5 - CATCTACAAGCAGTCACA - 3; Reverse 5 - GCAGTGCTCGCTTAGTGC - 3. The amplified product size is 442 bp. The following oligonucleotide sequence can be used as an internal probe to detect the amplified product: 5 - ATCCACTACAACTACATG - 3. Insert was isolated from php53B plasmid DNA (ATCC 57255) and subcloned into a vector allowing RNA transcription. |
| References： | Zakut-Houri R, et al. Human P53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4: 1251-1255, 1985. PubMed: 4006916 Robert McLinden, personal communication |