微信小陈
微信小章| 产品名称： | pFL46L |
|---|---|
| 商品货号： | TS166443 |
| Designations： | pFL46L |
| Depositors： | F Lacroute |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.8220000267028810 Vector: pFL46L (plasmid) Construction: pUC19 Marker(s):LEU2,ampR Construct size (kb): 7.822000026702881 Features: marker(s): ampR, LEU2 replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.8; BglII--4.9, 2.9; ClaI--3.7, 2.3, 1.9. Contrary to published data, this vector does not permit visual detection of recombinants using the assay for alpha complementation. Shows increased stability because of the presence of STB. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 6.3. YE-type high copy number shuttle vector. The order of the major features in this plasmid is: NotI/MCS/HindIII - LEU2 - STB -2 micron ori - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470 Struhl K, et al. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. USA 76: 1035-1039, 1979. PubMed: 375221 Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589 Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730 Francois Lacroute, personal communication |
| Shipped： | freeze-dried |