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| 产品名称： | hPKCalpha-GF29 |
|---|---|
| 商品货号： | TS166503 |
| Designations： | hPKCalpha-GF29 |
| GenBank Number： | X52479 |
| Species： | Homo sapiens, human |
| Depositors： | H Hug |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.244999885559082 Tissue: acute T cell leukemia, Jurkat cell line Gene product: protein kinase C, alpha PKCA |
| Insert Size (kb)： | 2.245 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.3; BamHI--4.5, 0.65, 0.25; BglI--2.7, 1.3 (doublet); NcoI--4.2, 1.2; PvuII--2.4, 1.4, 0.75, 0.6. The insert contains the following restriction sites (bp from the 5 end): BamHI--585, 796; BglI--965; NcoI--26, 1178; PstI--178; PvuII--1233, 1951; SmaI--44. Contains the complete coding sequence (nt 28-2046). The insert is oriented such that transcription from T7 gives the sense strand and from T3 gives the antisense strand. |
| References： | Finkenzeller G, et al. Sequence of human protein kinase C alpha. Nucleic Acids Res. 18: 2183, 1990. PubMed: 2336401 Hubert Hug, personal communication |