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| 产品名称： | pAQP4 |
|---|---|
| 商品货号： | TS166570 |
| Designations： | pAQP4 |
| GenBank Number： | U14007 |
| Species： | Rattus norvegicus, rat |
| Depositors： | P Agre, GM Preston |
| Vector： | Construct size (kb): 4.599999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: rat Gene symbol: AQP4 Gene name: aquaporin 4 Contains complete coding sequence?: N Tissue: brain Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 1.6 Cross references: DNA Seq. Acc.: U14007 Nucleotides 1-1588 of the insert correspond to nucleotides 7-1595 of U14007. Insert lengths(kb): 1.600000023841858 Tissue: brain Gene product: aquaporin 4 AQP4 |
| Insert Size (kb)： | 1.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.6; HindIII--4.2, 0.3; SmaI--3.7, 0.85; KpnI--4.6; SstI--3.8, 0.8. Encodes a mercury-resistant water channel expressed primarily in rat adult brain. |
| References： | Jung JS, et al. Molecular characterization of an aquaporin cDNA from brain: candidate osmoreceptor and regulator of water balance. Proc. Natl. Acad. Sci. USA 91: 13052-13056, 1994. PubMed: 7528931 |