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| 产品名称： | pPE1000 |
|---|---|
| 商品货号： | TS166701 |
| Designations： | pPE1000 |
| Depositors： | PM Ealing |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.2500000000000000 Vector: pPE1000 (phagemid) Promoters: Promoter duplicated CaMV35S Construction: pGEM-9Zf derivative pART7 Marker(s):ampR Construct size (kb): 4.25 Features: marker(s): ampR promoter: duplicated CaMV35S replicon: pMB1, f1 ori MCS: NcoI...BglII MCS: SalI...EcoRI transcription terminator: from the nopaline synthase (NOS) gene epitope tag: HA1 epitope (YPYDVPDYA) |
| Applications： | encodes an epitope tag for protein isolation or monitoring vector useful for cloning PCR products |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--2.9, 1.3; NotI--2.9, 1.3; XhoI--4.2. Restriction sites flanking the 2X35S/EPITOPE/NOS 3 cassette allow excision with either NotI for subcloning into the binary vector pART27 or KpnI for subcloning into the binary v ector pRD400. There are also unique SacI and XhoI sites for either directional subcloning or insertion of other plant transgenes before subcloning. The vector contains a specialized cassette allowing the in-frame N- or C-terminal fusion of a polymerase chain reaction (PCR)-amplified mature protein coding sequence from any gene to a sequence encoding an epitope of the influenza hemagglutanin a-chain. The vector was constructed using the pGEM-9Zf derivative pART7 from the vector PJIT166 and the 3 transcription terminator from the nopaline synthase (NOS) gene subcloned from the vector pCaGUS. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Hancock KR, et al. pPE1000: a versatile vector for the expression of epitope-tagged foreign proteins in transgenic plants. BioTechniques 22: 861-865, 1997. PubMed: 9149864 |
| Shipped： | freeze-dried |