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微信小章| 产品名称： | Human adenovirus 5 |
|---|---|
| 商品货号： | TS166780 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus C |
| Agent： | Human adenovirus 5 |
| Strain： | Adenovirus Type 5 Reference Material (The Adenovirus Reference Material (ARM) consists of purified Adenovirus, Type 5 (wild type adenovirus, see ATCC VR-5) formulated as a sterile liquid in 20 mM TRIS, 25 mM NaCl, 2.5% glycerol, pH 8.0 at room temperature, and stored frozen at -70°C. The configuration is 0.5-mL in a Type II glass vial with a Teflon-coated gray butyl stopper, plus an aluminum seal and crimp closure.) |
| Applications： | Reference material Respiratory research |
| Positive for Mycoplasma： | No |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (Vials have not been tested sufficiently at liquid nitrogen (vapor or liquid) temperatures. At this time it is not certain whether or not the seal will be maintained at cryogenic temperatures.) |
| Genome Sequenced Strain： | Human adenovirus C serotype 5, complete genome. |
| Comments： | For additional information on handling the Adenovirus Type 5 Reference Material,xa0view the ARMWG Handling Guidexa0located on the ATCC website at www.atcc.org. |
| Growth Conditions： | Temperature: 37°C |
| Name of Depositor： | Adenovirus Reference Material Working Group (ARMWG) |
| Special Collection： | NCRR Contract |
| References： | Hutchins B Development of a reference material for characterizing adenovirus vectors Bioprocess. J. 125-28, 2002 Available from: BioProcessing Journal Hutchins B, et al. Working toward an adenoviral vector testing standard. Mol. Ther. 2: 532-534, 2000. PubMed: 11124052 Nyberg-Hoffman C, et al. Sensitivity and reproducibility in adenoviral infectious titer determination.. Nat. Med. 3: 808-811, 1997. PubMed: 9212113 Smith GJ III, et al. Fast and accurate method for quantitating E. coli host-cell DNA contamination in plasmid DNA preparations.. BioTechniques 26: 518-526, 1999. PubMed: 10090994 Hoffman K. Strategies for host cell protein analysis. BioTechniques 13: 38-45, 2000. Vallekamp G, et al. Empty capsids in column-purified recombinant adenovirus preparations.. Hum. Gene Ther. 12: 1923-1936, 2001. PubMed: 11589834 Huyghe BG, et al. Purification of a type 5 recombinant adenovirus encoding human p53 by column chromatography.. Hum. Gene Ther. 6: 1403-1416, 1995. PubMed: 8573613 Shabram PW, et al. Analytical anion-exchange HPLC of recombinant type-5 adenoviral particles.. Hum. Gene Ther. 8: 453-465, 1997. PubMed: 9054520 Lehmberg E, et al. Reversed-phase high-performance liquid chromatographic assay for the adenovirus type 5 proteome.. J. Chromatogr. B Biomed. Sci. Appl. 732: 411-423, 1999. PubMed: 10517364 Obenauer-Kutner LJ, et al. The use of field emission scanning electron microscopy to assess recombinant adenovirus stability. Hum. Gene Ther. 13: 1687-1696, 2002. PubMed: 12396622 Callahan JD. Statistical analysis of adenovirus reference material assay results: determination of particle concentration and infectious titer.. La Jolla, CA: Callahan Associates Inc.; 2002. The Adenovirus Reference Material (ARM) consists of purified Adenovirus, Type 5 (wild type adenovirus, see ATCC VR-5) formulated as a sterile liquid in 20 mM TRIS, 25 mM NaCl, 2.5% glycerol, pH 8.0 at room temperature, and stored frozen at -70°C. The configuration is 0.5-mL in a Type II glass vial with a Teflon-coated gray butyl stopper, plus an aluminum seal and crimp closure. Vials have not been tested sufficiently at liquid nitrogen (vapor or liquid) temperatures. At this time it is not certain whether or not the seal will be maintained at cryogenic temperatures. |
| Cross References： | Complete Genome (GenBank) : AY339865 Human adenovirus C serotype 5, complete genome. |