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| 产品名称： | pDMR |
|---|---|
| 商品货号： | TS166818 |
| Designations： | pDMR |
| GenBank Number： | M23412 |
| Species： | Drosophila melanogaster, fruit fly |
| Depositors： | JC Venter |
| Vector： | Construct size (kb): 7.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.800000190734863 Gene product: cholinergic receptor, muscarinic CHRM* |
| Insert Size (kb)： | 4.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.8, 3.5, 0.6; BamHI--6.0, 2.4, 0.3; HindIII--6.3, 1.6; SmaI--7.8; EcoRI/SmaI--7.8; EcoRI--7.8; EcoRI/PstI--3.4, 3.0, 0.6 (doublet), 0.3. The insert contains the following restriction sites (nt position according to the published sequence): PstI--434, 700, 1210, 1711; BamHI--2596; HindIII--3316. A full-length cDNA clone which includes approximately 0.3 kb 5 and 2.1 kb 3 untranslated sequence. Constructed by blunt-ending an EcoRI fragment before inserting it into the vector. |
| References： | Onai T, et al. Chromosome localization of a Drosophila muscarinic cholinergic receptor. FEBS Lett. 255: 219-225, 1989. PubMed: 2507354 J C Venter, personal communication |