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| 产品名称： | pCMI37 |
|---|---|
| 商品货号： | TS166955 |
| Designations： | pCMI37 |
| Species： | Homo sapiens, human |
| Applications： | RFLPs are observed under normal hybridization stringency. |
| Vector： | Construct size (kb): 7.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 3.5 Gene product: DNA Segment, single copy D7S368 Alleles: A1, A2, C1, C2 |
| Insert Size (kb)： | 3.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul, |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--7.8; EcoRI--7.8; PvuII/HindIII--3.85, 3.35, 0.55; PvuII/PstI--3.45, 2.65, 1.35; ClaI--7.8; EcoRI/HindIII--3.9, 3.7; BamHI--uncut. Maps near the centromere. Polymorphisms are best resolved on 1.2% agarose gels with bromphenol blue run longer than 14 cm. RFLPs are observed under normal hybridization stringency. Washes should be done at 0.1X SSC, 0.1% SDS, 65C. EcoRI, HindIII, PvuII, BglII, PstI and BamHI neither detect the site polymorphisms nor resolve the VNTR polymorphism adequately. |
| References： | Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pCMI37) on chromosome 7 D7S368. Nucleic Acids Res. 16: 4192, 1988. PubMed: 2897676 Yusuke Nakamura, personal communication Peter J OConnell, personal communication |