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| 产品名称： | pKO11 |
|---|---|
| 商品货号： | TS167014 |
| Designations： | pKO11 |
| Depositors： | M Rosenberg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.9000000953674320 Vector: pKO11 (plasmid) Promoters: Promoter none Construction: pKO1 (ATCC 37126) Marker(s):ampR Construct size (kb): 3.900000095367432 Features: insert detection: galK marker(s): ampR promoter: none replicon: pMB1 |
| Applications： | promoter-cloning vector |
| Comments： | This is a promoter cloning vector using expression of the promoterless galK gene for positive or color (MacConkey galactose indicator plates) selection. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | McKenney K, et al. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif. Anal. 2: 383-415, 1981. PubMed: 6101056 Adams CW, Hatfield GW. Effects of promoter strengths and growth conditions on copy number of transcriptions-fusion vectors. J. Biol. Chem. 259: 7399-7403, 1984. PubMed: 6736011 Nierman WCVectors for cloning promoters and terminatorsIn: Nierman WCVectors: a survey of molecular cloning vectors and their usesBostonButterworthpp. 153-177, 1988 |
| Shipped： | freeze-dried |