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| 产品名称： | YRp7 pRB16 |
|---|---|
| 商品货号： | TS167108 |
| Designations： | YRp7 pRB16 |
| Depositors： | D Botstein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.817 Vector: YRp7 (plasmid) Promoters: none Construction: pBR322, lambdagt-Sc4101 Marker(s):TRP1,ampR,tetR Construct size (kb): 5.817 Features: marker(s): TRP1 marker(s): ampR marker(s): tetR promoter: none replicon: ARS1 replicon: ROP replicon: pMB1 terminator: none enhancer: none |
| Applications： | YR-type (replicating) shuttle vector shuttle vector |
| Comments： | A general purpose replicating Saccharomyces cerevisiae/Escherichia coli shuttle vector. YRp7 was constructed by inserting a 1.4 kb EcoRI fragment containing TRP1 and ARS1 from lambdagt-Sc4101 into pBR322. The ARS1 fragment is in a 0.85 kb EcoRI-HindIII subfragment. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030 Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |