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| 产品名称： | pUCD2 plasmid in E. coli |
|---|---|
| 商品货号： | TS167118 |
| Designations： | pUCD2 plasmid in E. coli |
| Depositors： | CI Kado |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 13.0 Vector: pUCD2 (plasmid) Promoters: Promoter none Construction: pBR322, pSal151 Marker(s):ampR,kanR,spcR,tetR Construct size (kb): 13.0 Features: marker(s): ampR, kanR, spcR, tetR promoter: none replicon: pMB1, pSa151 terminator: none enhancer: none |
| Applications： | shuttle vector |
| Comments： | A broad host range amplifiable cloning vector. This is a broad host range amplifiable cloning vector for use in complementation studies of cloned inserts in Agrobacterium tumefaciens. It was constructed by fusing pBR322 with pSAl51 plasmid and was shown to have lost 2.5 kb on each side of the BglII site giving it a positive vir region complementation in Agrobacterium tumafaciens. Restriction digests of the clone give the following sizes (kb): PstI--13; EcoRV--13; KpnI--13; SalI--13. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Close TJ, et al. Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58. Plasmid 12: 111-118, 1984. PubMed: 6095350 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the cosmid |