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| 产品名称： | pSI4 |
|---|---|
| 商品货号： | TS167192 |
| Designations： | pSI4 |
| Depositors： | JR Broach |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.8999996185302700 Vector: pSI4 (plasmid) Construction: pJDB219, pBR322 Marker(s):ampR,tetR,LEU2-d Construct size (kb): 11.89999961853027 Features: marker(s): ampR, tetR, LEU2-d replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector shuttle vector vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--12.0; EcoRI--4.4, 4.1, 2.6, 0.8; HindIII--4.6, 3.4, 2.7, 1.3; PstI--12.0. LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine). High-copy YE-type shuttle vector. Consists of the entire 2 micron circle genome cloned, at the EcoRI site of the small unique region, into the EcoRI site of pBR322. The leu2-d sequence (approximately 1.4 kb) is inserted into the PstI site of the 2 micron circle. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781 Broach JR. Construction of high copy yeast vectors using 2-micron circle sequences. Methods Enzymol. 101: 307-325, 1983. PubMed: 6310334 |
| Shipped： | freeze-dried |