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微信小章| 产品名称： | G1B |
|---|---|
| 商品货号： | TS167260 |
| Organism： | Clarias batrachus, walking catfish |
| Tissue： | gill |
| Product Format： | frozen |
| Morphology： | pleomorphic |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The G1B cell line was derived from normal gill tissue taken from a healthy adult walking catfish. |
| Comments： | The G1B cell line was derived from normal gill tissue taken from a healthy adult walking catfish. The cells support the in vitro growth of Amyloodinium ocellatum, a dinoflagellate that infects the gills and skin of both marine and brackish water fishes. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 25°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | EJ Noga |
| Deposited As： | Clarias batrachus |
| References： | Noga EJ, Hartmann JX. Establishment of walking catfish (Clarias batrachus) cell lines and development of a channel catfish (Ictalurus punctatus) virus vaccine. Can. J. Fish. Aquat. Sci. 38: 925-930, 1981. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |