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| 产品名称： | pDP1115 |
|---|---|
| 商品货号： | TS167290 |
| Designations： | pDP1115 |
| Species： | Mus musculus, mouse |
| Depositors： | DC Page |
| Vector： | Construct size (kb): 9.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 6.800000190734863 Tissue: newborn liver Gene product: zinc finger protein, X-linked Zfx |
| Insert Size (kb)： | 6.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.9, 2.8; HindIII--7.4, 0.96, 0.8, 0.58; BamHI--3.8, 3.4, 2.0, 0.8; PstI--4.7, 3.0, 1.2, 0.8; AccI--4.4, 2.1, 1.18, 0.8 (doublet), 0.6; HincII--3.9, 1.7 (doublet), 1.3, 1.07; SalI--9.8. The insert contains the following restriction sites (in kb from the 5 end): EcoRI--2.7; HindIII--4.5, 5.25, 6.2. The complete coding sequence is included in the 5 EcoRI fragment of 2.7 kb, The 2.7 kb EcoRI fragment and the 1.8 kb EcoRI/HindIII fragment detect mRNA of 7.5 and 6.0 kb. The 3 0.6 kb HindIII fragment detects only the 7.5 kb mRNA. The clone was derived from the 7.5 kb mRNA. which detects genomic EcoRI fragments of 2.1 kb (Zfa) and 1.9 kb (Zfx). The 1.8 kb EcoRI/HindIII fragment from the insert detects genomic EcoRI fragments of 8.2 kb (Zfa) and 5.3 kb (Zfx). |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Mardon G, et al. Mouse Zfx protein is similar to Zfy-2: each contains an acidic activating domain and 13 zinc finger. Mol. Cell. Biol. 10: 681-688, 1990. PubMed: 2105457 |