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微信小章| 产品名称： | Trypanosoma mega Dutton and Todd |
|---|---|
| 商品货号： | TS167403 |
| Application： | Vector borne research |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | toad, Bufo regularis, Africa |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Phylogenetic relationships among anuran trypanosomes Polyamine function Cysteine and metalloproteinase activities Genus identification based on specific 18SRibosomal RNA sequence Polyamines Karyotype analysis of anuran trypanosomes enzymes of ornithine-arginine metabolism Glycosphingolipid Proteolytic activities Acetylornithinase and ornithine acetyltransferase |
| Medium： | ATCC® Medium 1011: Diphasic blood agar medium |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Subcultivation： | Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Name of Depositor： | HN Guttman |
| Chain of Custody： | ATCC < |
| References： | Clark CG, et al. Phylogenetic relationship among anuran Trypanosoma as revealed by riboprinting. J. Eukaryot. Microbiol. 42: 92-96, 1995. PubMed: 7728144 Nature 178: 308-309, 1956. Bacchi CJ, et al. Polyamine function in trypanosomatids: activation of cytoplasmic alpha-glycerophosphate dehydrogenase by cationic trypanocides. Adv. Polyamine Res. 2: 129-138, 1978. Lopez TD. Volumen celular y adn de Tripanosomas. Acta Macarao 1(1): 1-12, 1973. Lopez TD. Volumen celular y contenido de adn de Trypanosoma lewisi y T. mega in vitro. Acta Macarao 1(1): 13-20, 1973. Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304 Branquinha MH, et al. Ubiquity of cysteine-and metalloproteinase activites in a wide range of trypanosomatids. J. Eukaryot. Microbiol. 43: 131-135, 1996. PubMed: 8720943 Uliana SR, et al. Leishmania: genus identification based on a specific sequence of the 18S ribosomal RNA sequence. Exp. Parasitol. 72: 157-163, 1991. PubMed: 2009920 Bacchi CJ, et al. Polyamines in trypanosomatids. J. Bacteriol. 131: 657-661, 1977. PubMed: 885842 Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594 Lun ZR, Desser SS. Karyotype analysis of anuran trypanosomes by pulsed-field gradient gel electrophoresis. J. Parasitol. 81: 1018-1020, 1995. PubMed: 8544044 Camargo EP, et al. Trypanosoma spp., Leishmania spp. and Leptomonas spp.: enzymes of ornithine-arginine metabolism. Exp. Parasitol. 46: 141-144, 1978. PubMed: 569593 Vermelho AB, et al. Isolation and characterization of a neutral glycosphingolipid from the epimastigote form of Trypanosoma mega. J. Protozool. 33: 208-213, 1986. PubMed: 3735150 |
| Cross References： | Nucleotide (GenBank) : AJ223567 Trypanosoma mega, small subunit ribosomal RNA gene. |