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微信小章| 产品名称： | 36B4 |
|---|---|
| 商品货号： | TS167428 |
| Designations： | 36B4 |
| Species： | Homo sapiens, human |
| Depositors： | P Chambon |
| Applications： | Used as a control in RNase protection experiments to study the regulation by estradiol of transcription. |
| Vector： | Construct size (kb): 5.369999885559082 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.067999958992004 Tissue: breast cancer MCF-7 cell line Gene product: ribosomal phosphoprotein P0(acidic ribosomal protein P0 (L10E)) RPP0* |
| Insert Size (kb)： | 1.068 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; PstI--4.4, 0.7, 0.2, 0.1; HindIII--5.4; BamHI--5.4; SalI--5.4. The insert contains the following restriction sites (nt from one end): PstI--708, 928. Expressed constitutively, and not stimulated by estradiol. Used as a control in RNase protection experiments to study the regulation by estradiol of transcription. |
| References： | Masiakowski P, et al. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res. 10: 7895-7903, 1982. PubMed: 6897676 Laborda J. 36B4 cDNA used as an estradiol-independent mRNA control is the cDNA for human acidic ribosomal phosphoprotein PO. Nucleic Acids Res. 19: 3998, 1991. PubMed: 1861990 Brown AM, et al. Activation of pS2 gene transcription is a primary response to estrogen in the human breast cancer cell line MCF-7. Proc. Natl. Acad. Sci. USA 81: 6344-6348, 1984. PubMed: 6593703 Rio MC, et al. Specific expression of the pS2 gene in subclasses of breast cancers in comparison with expression of the estrogen and progesterone receptors and the oncogene ERBB2. Proc. Natl. Acad. Sci. USA 84: 9243-9247, 1987. PubMed: 3321071 Pierre Chambon, personal communication |