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| 产品名称： | pBSL119 |
|---|---|
| 商品货号： | TS167447 |
| Designations： | pBSL119 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.8420000076293950 Vector: pBSL119 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pBluescript Marker(s):ampR,cmlR Construct size (kb): 4.842000007629395 Features: marker(s): ampR other: omega element promoter for in vitro transcription: T7 (phi10) replicon: f1 replicon: pMB1 MCS: KpnI...SacI MluI MCS: MluI KpnI...SacI coding sequence: cmlR (cat) |
| Applications： | contains easily purifiable cassette(s) for construction omega-cat |
| Comments： | Restriction digests of the clone give the following sizes (kb): MluI--3.1, 1.8; EcoRI--2.9, 1.9; BamHI--2.9, 1.8. Contains an omega-Cm element acetyltransferase (cmlR) and multiple cloning sites duplicated in tandem orientation. Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA. The EcoRI site was removed. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718 |
| Shipped： | freeze-dried |