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| 产品名称： | HO-poly-HO |
|---|---|
| 商品货号： | TS167457 |
| Designations： | HO-poly-HO |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.5999999046325680 Vector: HO-poly-HO (plasmid) Promoters: Promoter HO Construction: pUC21 Marker(s):ampR Construct size (kb): 4.599999904632568 Features: promoter: HO |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--4.6; NotI--3.0, 1.6. A 912 bp PCR product with HindIII and BsiWI ends and containing HO promoter sequences (-1720 to -1814 upstream from the ATG) was inserted into the HindIII-BsiWI digested pUC21-NotI. A 507 bp PCR product with EcoRI and SfiI ends and containing 3 end sequences of HO gene (1199 to 1699 downstream from the ATG) was inserted into the EcoRI-SfiI digested pUC21-NotI. This plasmid allows targeted integration at HO locus. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C Protocol: (LB + amp) |
| References： | Voth WP. Yeast vectors for integration at the HO locus.. Nucleic Acids Res. 29(12): E59-9, 2001. PubMed: 11410682 David J Stillman, personal communication |
| Shipped： | freeze-dried |