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| 产品名称： | Human rhinovirus 39 |
|---|---|
| 商品货号： | TS167495 |
| Classification： | Picornaviridae, Enterovirus, Rhinovirus A |
| Deposited As： | Rhinovirus 39 |
| Agent： | Human rhinovirus 39 |
| Strain： | 209 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Growth not inhibited by IUDR. Stabilized by 1 M MgCl2. Does not hemagglutinate rat, rhesus monkey, or human blood type O. Non-pathogenic for suckling mice. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | H1-HeLa (ATCC® CRL-1958™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified atmosphere while rocking continuously. End adsorption by adding virus growth medium. Incubation: 1-7 days while rocking continuously |
| Name of Depositor： | MA Mufson, National Institute of Allergy and Infectious Diseases; RM Conant, VV Hamparian, The Ohio State University |
| Source： | Nasal washing from an adult female, North Carolina |
| References： | Mufson MA, et al. A description of six new Rhinoviruses of human origin. Am. J. Epidemiol. 81: 32-43, 1965. PubMed: 14246079 |