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微信小章| 产品名称： | LL288 |
|---|---|
| 商品货号： | TS167574 |
| Designations： | LL288 |
| GenBank Number： | U30610 |
| Species： | Homo sapiens, human |
| Depositors： | LL Lanier |
| Vector： | Construct size (kb): 4.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pJFE14 Intact vector size: 3.500 Type of vector: plasmid Vector end: BstXI Vector end: NotI Cloning sites: BstXI NotI SacII SfiI EcoRI Polylinker sites: BstXI NotI SacII SfiI EcoRI Construction: pcDL-SRalpha296 Host range: mammalian cells; Escherichia coli Features (with orientation and position when available): replicon: SV40 promoter for expression: SV40 early region enhancer: HTLV-1 R-U5 segment of LTR MCS: BstXI...EcoRI terminator: SV40 late region polyadenylation signal replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CD94 Gene name: CD94 antigen (NK/T-cell C-type lectin receptor) ORFs seq. position: GDB probe: GDB:696221 () Contains complete coding sequence?: Y Chromosome: 12; Localization: 12 p13 Tissue: polyclonal NK cell line Type of DNA: cDNA Insert 5 end: Modification: BstXI linker Insert 3 end: Modification: NotI linker Insert size (kb): 0.8 Cross references: DNA Seq. Acc.: U30610 Nucleotides 1-834 of the insert correspond to nucleotides 1-834 of U30610. Insert lengths(kb): 0.800000011920929 Tissue: polyclonal NK cell line Gene product: CD94 antigen (NK/T-cell C-type lectin receptor) CD94 |
| Insert Size (kb)： | 0.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BstXI--4.2; BstXI/NotI--3.4, 0.84; NotI--4.2. The insert contains the following restriction sites (approximate kb from the 5 end): KpnI--0.28; SacI--0.41. |
| References： | Chang C, et al. Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily. Eur. J. Immunol. 25: 2433-2437, 1995. PubMed: 7589107 Lanier LL, et al. Human NKR-P1A. A disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes. J. Immunol. 153: 2417-2428, 1994. PubMed: 8077657 Takebe Y, et al. SRalpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol. 8: 466-472, 1988. PubMed: 2827008 |