微信小陈
微信小章
产品简介
购买须知
| 产品名称： | RA-1.2 |
|---|---|
| 商品货号： | TS167602 |
| Designations： | RA-1.2 |
| GenBank Number： | U31346 |
| Species： | Homo sapiens, human |
| Depositors： | H Menard |
| Applications： | This clone expresses a fusion protein of GST and a truncated calpastatin. |
| Vector： | Construct size (kb): 4.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC19 Intact vector size: 2.686 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: EcoRI...HindIII, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: X02514 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CAST Gene name: calpastatin Contains complete coding sequence?: N Chromosome: 5; Localization: 5 q15-q21 Tissue: placenta Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.323 Cross references: DNA Seq. Acc.: U31346 Nucleotides 1-1323 of the insert correspond to nucleotides 1-1323 of U31346. Insert lengths(kb): 1.322999954223633 Tissue: placenta Gene product: calpastatin CAST |
| Insert Size (kb)： | 1.323 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.3; BamHI--4.0; HindIII--3.0, 0.65, 0.35; PstI--3.1, 0.9; EcoRI/PstI--2.7, 0.9, 0.5. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.30,0.87,1.15; PstI 0.42. This clone expresses a fusion protein of GST and a truncated calpastatin. This calpastatin begins at nt1677 of D16217 and continues to the poly(A) tail, but the 3 UTR is different. |
| References： | Despres N, et al. Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera. J. Clin. Invest. 95: 1891-1896, 1995. PubMed: 7706496 |