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| 产品名称： | pRS422 |
|---|---|
| 商品货号： | TS167676 |
| Designations： | pRS422 |
| GenBank Number： | U93719 |
| Depositors： | JD Boeke |
| Applications： | vector permitting visual detection of recombinants phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase |
| Vector： | Intact vector size: 6.868
Type of vector: phagemid Cloning sites: SacI SacII EagI NotI SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI ApaI KpnI Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI ApaI KpnI Construction: pRS402, pRS421 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): ADE2, →, 575-2290 replicon: f1, ←, 2495-2951 promoter for in vitro transcription: T7, →, 3121-3140 MCS: KpnI...SacI, →, 3148-3250 promoter for in vitro transcription: T3, ←, 3267-3286 coding sequence: lacZ, ←, 2952-3311 promoter: lac, ←, 3356-3384 replicon: pMB1, 3710-3710 marker(s): ampR, ←, 4468-5328 replicon: 2 micron, 5460-6801 Vector: pRS422 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS402, pRS421
Marker(s): ampR,ADE2
Construct size (kb): 6.868
Features: marker(s): ADE2 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: 2 micron replicon: f1 replicon: pMB1 MCS: KpnI...SacI coding sequence: lacZ |
| Insert： | Target Gene: , phosphoribosylaminoimidazole succinocarboxamide synthetase |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | Component of: ATCC 87480
Component of: ATCC 87530
Component of: ATCC 87535 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.8; BglII--4.6, 2.2; EcoRI--6.8. ade2 phenotype produces red colonies when grown on adenine containing media. This vector is useful for gene knockout experiments in hosts with a non-revertable ade2 auxotrophic marker gene mutation. |
| References： | Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |