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| 产品名称： | pGB42 |
|---|---|
| 商品货号： | TS167693 |
| Designations： | pGB42 |
| GenBank Number： | M11080 |
| Species： | Mus musculus, mouse |
| Depositors： | Genzyme Corp., G Barsomian, Genzyme Corp. |
| Applications： | in another host, produces protein glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.799999952316284 Gene product: dihydrofolate reductase GBA Target Gene: glucosidase, beta; acid |
| Insert Size (kb)： | 1.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BglII--6.0; HindIII--6.0. The glucocerebrosidase insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.83; PstI--0.27, 0.56; SalI--1.25; BamHI--1.48. Contains the complete coding sequence for glucocerebrosidase (nt 6-1556), plus 290 nt at the 3 end. Corresponds approximately to nt 490-2336 of the sequence record, with a modification at the 5 end from CCCACATGG to CCACCATGG. The vector was derived from pSV2-dhfr by adding the adenovirus promoter and removing the poison sequence from the pBR322 portion. Permits bicistronic expression, with glucocerebrosidase being translated before dhfr, resulting in greater expression of the insert sequence relative to dhfr. The order of the major features in the plasmid is: pMB1 ori - SV40 enhancer - Adenovirus major late promoter - XhoI - BglII - glucocerebrosidase insert - dihydrofolate reductase - SV40 polyadenylation - ampR. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Rassmussen J, et al. Enzymatically active recombinant glucocerebrosidase. US Patent 5,236,838 dated Aug 17 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |