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| 产品名称： | p101JdeltaE5.0 p101Jdelta1E5.0, p101delta1 5.0E |
|---|---|
| 商品货号： | TS167698 |
| Designations： | p101JdeltaE5.0 p101Jdelta1E5.0, p101delta1 5.0E |
| Species： | Homo sapiens, human |
| Depositors： | Temple University, CM Croce, Temple University |
| Applications： | Has been used to detect t(10;14)(q24;q11) translocations associated with certain acute T-cell leukemias based on an alteration in the size of the BamHI fragment detected. |
| Vector： | Construct size (kb): 7.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 5.0 Tissue: placenta Gene product: T cell receptor, delta (V,D,J,C) TCRD |
| Insert Size (kb)： | 5.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.6; EcoRI--5.0, 2.8. The insert contains the following restriction sites (approximate kb from the 5 end): SacI--1.1, 1.8, 3.0; HindIII--3.5, 4.0. The MOLT-4 cell line (ATCC CRL-1582) is deleted for this fragment. Detects the following human genomic germline restriction fragments (kb): BamHI--16.5; HindIII--5.8, 3.2. Has been used to detect t(10;14)(q24;q11) translocations associated with certain acute T-cell leukemias based on an alteration in the size of the BamHI fragment detected. Corresponds to the EcoRI fragment between Jdelta1 and Jdelta2. |
| References： | Kagan J, et al. Clustering of breakpoints on chromosome 10 in acute T-cell leukemias with the t(10;14) chromosome translocation. Proc. Natl. Acad. Sci. USA 86: 4161-4165, 1989. PubMed: 2542965 Croce CM. Molecular probing for human T-cell leukemia and lymphoma. US Patent 5,198,338 dated Mar 30 1993 Croce CM. TCL-5 gene rearrangement involved in T-cell leukemia and melanoma. US Patent 5,242,795 dated Sep 7 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |