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| 产品名称： | hARb CL7b-AR 160-911 |
|---|---|
| 商品货号： | TS167701 |
| Designations： | hARb CL7b-AR 160-911 |
| GenBank Number： | M73069 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein androgen receptor (dihydrotestosterone receptor; testicular feminization) |
| Vector： | Construct size (kb): 8.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pKCR-2 Intact vector size: 5.700 Type of vector: plasmid Vector end: EcoRI Modification: blunt ended Vector end: EcoRI Modification: blunt ended Cloning sites: BamHI, EcoRI Polylinker sites: Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: AR Other gene symbols: DHTR Genomic copy number: unique Gene name: androgen receptor (dihydrotestosterone receptor; testicular feminization) Contains complete coding sequence?: U Chromosome: X; Localization: X q11.2-q12 Tissue: testis Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 2.8 Cross references: DNA Seq. Acc.: M73069 Insert lengths(kb): 2.799999952316284 Tissue: testis Gene product: androgen receptor (dihydrotestosterone receptor; testicular feminization) AR Target Gene: androgen receptor (dihydrotestosterone receptor; testicular feminization) |
| Insert Size (kb)： | 2.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--9.0; EcoRI--6.5, 1.8, 0.58; HindIII--9.0; KpnI--6.5, 2.0, PstI--4.5, 2.1(doublet), 0.31. Restriction digests of the clone give the following sizes (kb): BamHI--9.0; EcoRI--6.5, 1.8, 0.58; HindIII--9.0; KpnI--6.5, 2.0; PstI--4.5, 2.1 (doublet), 0.31. Digestion with EcoRI yields two fragments of 2.0 and 0.5 kb. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.2, 0.4, 0.7, 0.75; EcoRI--1.9; HindIII--1.2; KpnI--1.0; NcoI--0.3, 1.75; PstI--0.5; PvuI--2.0; PvuII--0.05, 0.6; SacI--0.5, 1.2. Encodes aa 160-911 of the mutant form: K816I, D819F, E820F and Leu insertion at aa 821. Cross hybridizes with a 6.8 kb rat liver mRNA. pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals. |
| References： | Govindan MV. Specific region in hormone binding domain is essential for hormone binding and trans-activation by human androgen receptor. Mol. Endocrinol. 4: 417-427, 1990. PubMed: 2342476 |