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微信小章| 产品名称： | Human respiratory syncytial virus, Purified |
|---|---|
| 商品货号： | TS167712 |
| Classification： | Paramyxoviridae, Pneumovirus |
| Deposited As： | Respiratory syncytial virus |
| Agent： | Human respiratory syncytial virus, Purified |
| Strain： | A2 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 200 µL per vial |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | ATCC® VR-1540P™ is a purified virus product suspended in 200 µL of Hanks Balanced Salt Solution with Ca2+, Mg2+ and 25 mM HEPES Buffer. ATCC®xa0VR-1540™ is prepared from original isolate deposited as ATCC®xa0VR-1302™, which was grown in presence of neutralizing serum to remove contaminating adenovirus type 1. |
| Effect on Host： | CPE, syncytial formation, rounding, and sloughing |
| Recommended Host： |
HeLa (ATCC® CCL-2™)
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| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an MOI 0.1. Adsorb for 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3 to 10 days |
| Name of Depositor： | ATCC, L Potash |
| Source： | Lower respiratory tract of infant with bronchiolitis and bronchopneumonia, Melbourne, Australia, 1961 |
| References： | Cameron R, et al. Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302). Virus Res. 92: 151-156, 2003. PubMed: 12686423 Lewis FA, et al. A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Med. J. Aust. 2: 932-933, 1961. |