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lambdaD7 [pcD-GALT]

货号 TS167798
中文名称 null
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产品名称: lambdaD7 pcD-GALT
商品货号: TS167798
Designations: lambdaD7 pcD-GALT
GenBank Number:

M12961

Species: Homo sapiens, human
Depositors: M Lefranc, TH Rabbitts
Vector:
Construct size (kb): 45.29999923706055
Insert:
DNA: genomic
Insert lengths(kb): 15.80000019073486
Tissue: D-PLL cell line (B-prolymphocytic leukemia)
Gene product: T cell receptor, gamma, constant region C1 TCRGC1
Alleles: B1, D4, J2, D2, A1, A2, B2, G1, G2, J1, D1, D3
Insert Size (kb): 15.800
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried bacteria-free lysate
Comments:
Restriction digests of the clone give the following sizes (kb): XbaI--20.0, 11.0, 9.2, 3.2, 2.4; SacI--20.0, 9.2, 6.2 (doublet), 4.4; XhoI--20.0, 11.0, 9.2, 3.2, 3.1; SmaI--20.0, 11.0, 6.0, 5.8, 3.7; PvuI--23.0, 12.0, 8.2.
Contains germline DNA beginning about 1.9 kb upstream of Cgamma1 exon 2 and extending about 1.4 kb beyond Cgamma2 exon 1. Contains the allele with the polymorphic HindIII site in Jgamma2 missing.
Additional GenBank/EMBL accession numbers: M14997, M14998, M15002, X03435.
References:

Lefranc MP, Rabbitts TH. Two tandemly organized human genes encoding the T-cell gamma constant-region sequences show multiple rearrangement in difference T-cell types. Nature 316: 464-466, 1985. PubMed: 2991773

Lefranc MP, et al. Genetic polymorphism and exon changes of the constant regions of the human T-cell rearranging gene gamma. Proc. Natl. Acad. Sci. USA 83: 9596-9600, 1986. PubMed: 2879283

Marie-Paule Lefranc, personal communication