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| 产品名称： | p6a6 pVZ6a6x, rtFcgammaRalpha |
|---|---|
| 商品货号： | TS167856 |
| Designations： | p6a6 pVZ6a6x, rtFcgammaRalpha |
| GenBank Number： | M32062 |
| Species： | Rattus norvegicus, rat |
| Depositors： | DW Sears |
| Vector： | Construct size (kb): 4.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.340999960899353 Tissue: CRNK-16 cell line (LGL leukemia) Gene product: Fc gamma low affinity receptor III, alpha subunit (CD16) CD16 |
| Insert Size (kb)： | 1.341 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.25, 1.4; BamHI--3.5, 1.15; PvuII--2.9, 1.1, 0.65; SacI--3.6, 1.05. The insert contains the following sites (in bp from the 5 end): BamHI--195; NsiI--636; PvuII--499; SacI--290; SmaI--1013. The insert contains 49 bp 5 untranslated, a complete open reading frame (49-850) and 491 bp 3 untranslated sequence. The extracellular domain is encoded from 143-694, the transmembrane region from 695-850, and the cytoplasmic domain from 783-850. The insert is oriented in the + strand direction relative to the universal primer. |
| References： | Zeger DL, et al. Characterization and expression of an Fcgamma receptor cDNA cloned from rat natural killer cells. Proc. Natl. Acad. Sci. USA 87: 3425-3429, 1990. PubMed: 1692135 Duane W Sears, personal communication |