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| 产品名称： | pZilch |
|---|---|
| 商品货号： | TS167872 |
| Designations： | pZilch |
| Depositors： | C Hovens |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.0770001411438000 Vector: pZilch (phagemid) Promoters: Promoter CMV Construction: pCATCH Marker(s):ampR Construct size (kb): 6.0770001411438 Features: marker(s): ampR promoter: CMV promoter for in vitro transcription: SP6 promoter for in vitro transcription: T7 replicon: M13 replicon: SV40 ori replicon: pMB1 replicon: polyoma MCS: BamHI...XhoI MCS: BglII...SalI terminator: SV40 coding sequence: mutant pheS gene epitope tag: FLAG |
| Applications： | encodes removable tag for protein isolation expression vector vector permitting construction of fusion proteins vector permitting positive selection for inserts vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1. The vector was contructed by inserting a 1185 bp SmaI/BamHI fragment from pKSS encompassing the mutant pheS allele into the pCATCH and modified further to generate polylinker and to incorporate Kozak sequence and initiation ATG codon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Schneider S, et al. An epitope tagged mammalian/prokaryotic expression vector with positive selection of cloned inserts. Gene 197: 337-341, 1997. PubMed: 9332383 |
| Shipped： | freeze-dried |