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| 产品名称： | YEp358R |
|---|---|
| 商品货号： | TS167878 |
| Designations： | YEp358R |
| Depositors： | CJ Lusty |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.0000000000000000 Vector: YEp358R (plasmid) Promoters: Promoter none Construction: YEp358 (ATCC 37733), pUC18 Marker(s):URA3,ampR Construct size (kb): 8.0 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: none replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector promoter-cloning vector shuttle vector |
| Comments： | Cloning into the HindIII, SphI, PstI, SalI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-2; SphI-3; PstI-3; SalI-2; XbaI-2; BamHI-2; SmaI-3; KpnI-3; SacI-3; EcoRI-2. The SacI site is not unique. Restriction digests of the clone give the following sizes (kb): HindIII--8.0; EcoRI--8.0; PstI--8.0. One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37737 - 37739) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The sequence and reading frame of the multiple cloning sequence is: 5AA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CCC AGC TTC GAT CCC3, from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915 |
| Shipped： | freeze-dried |
| Shipping Information： | in E. coli containing the plasmid |