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| 产品名称： | pTRP56 |
|---|---|
| 商品货号： | TS167923 |
| Designations： | pTRP56 |
| Depositors： | A Miyajima |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.1999998092651370 Vector: pTRP56 (plasmid) Promoters: Promoter ADC1 Construction: YRp7, Saccharomyces cerevisiae TRP5 terminator Marker(s):TRP1,ampR,tetR Construct size (kb): 6.199999809265137 Features: marker(s): ampR, tetR, TRP1 promoter: ADC1 replicon: pMB1, ARS1 terminator: TRP5 enhancer: none |
| Applications： | YR-type (replicating) shuttle vector expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--6.2; BamHI--6.2. A cloning vector that uses the plasmid primer approach of Okayama & Berg to allow expression of cDNA clones in yeast. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Miyajima A, et al. Analysis of full-length cDNA clones carrying GAL1 of Saccharomyces cerevisiae: a model system for cDNA expression. Nucleic Acids Res. 12: 6397-6414, 1984. PubMed: 6206472 |
| Shipped： | freeze-dried |