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| 产品名称： | YRp14-trp1delta63 YRp14/trp1-delta63, p430 |
|---|---|
| 商品货号： | TS167959 |
| Designations： | YRp14-trp1delta63 YRp14/trp1-delta63, p430 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 8.3999996185302730 DESCRIPTION OF VECTOR: Intact vector size: 8.400 Type of vector: plasmid Cloning sites: Polylinker sites: Construction: YRp14 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): ampR, URA3, SUP11 replicon: pMB1, ARS1 Vector: YRp14-trp1delta63 (plasmid) Construction: YRp14 Marker(s):URA3,ampR,SUP11 Construct size (kb): 8.399999618530273 Features: marker(s): ampR, URA3, SUP11 replicon: pMB1, ARS1 |
| Applications： | vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.0, 1.6, 0.6; HindIII--8.4; BamHI--8.4; PstI--3.7, 3.4, 1.3; BglII--7.4, 1.1. A marker addition/transplacement vector. Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae. Constructed by inserting the equivalent of 2 genomic EcoRI/HindIII fragments (1.5 kb, centromere proximal and 0.8 kb, 3 end) from the TRP1 region into YRp14. Represents the deletion of a 0.6 kb EcoRI/HindIII fragment containing the promoter and the 5 portion of the gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 Philip Hieter, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |