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微信小章| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS167982 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | deltaH1 delta H1 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | F- delta(bio-uvrB) lacZam lacI lambda Nam7 Nam53 cI857 deltaH1(cro-F-A-J-b2) |
| Type Strain： | no |
| Comments： | This is temperature sensitive. Grow at 30C. A defective lambda lysogen carrying the cI857 mutation. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 30.0°C
Atmosphere: aerobic |
| Name of Depositor： | H Bernard |
| References： | Bernard HU, et al. Construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda pL promoter. Gene 5: 59-76, 1979. PubMed: 372049 Bernard HU, Helinski DR. Use of the lambda phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles. Methods Enzymol. 68: 482-492, 1979. PubMed: 232223 Castellazzi M, et al. Isolation and characterization of deletions in bacteriophage lambda residing as prophage in E. coli K12. Mol. Gen. Genet. 117: 211-218, 1972. PubMed: 5057547 Riordan JR, et al. Amplification of P-glycoprotein genes in multidrug-resistant mammalian cell lines. Nature 316: 817-819, 1985. PubMed: 2863759 Hans-Ulrich Bernard, personal communication |