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| 产品名称： | pSM257 |
|---|---|
| 商品货号： | TS168022 |
| Designations： | pSM257 |
| GenBank Number： | M25247 |
| Species： | Pseudomonas sp. |
| Depositors： | G Grandi |
| Applications： | produces protein isoamylase |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: genomic Insert lengths(kb): 3.339999914169312 Gene product: isoamylase iam Target Gene: isoamylase |
| Insert Size (kb)： | 3.340 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.7; HindIII--6.1; PstI--6.0; BamHI--6.1. This clone was previously deposited as ATCC 67474. The insert contains the following restriction sites (nt position according to the published sequence): AatII--618; BamHI--688; EcoRI--973. Secretes isoamylase regulated by a promoter in the insert. The recombinant protein is recognized by anti-SMP1 isoamylase antibodies. |
| References： | Tognoni A, et al. Cloning and nucleotide sequence of the isoamylase gene from a strain of Pseudomonas sp.. J. Gen. Microbiol. 135: 37-45, 1989. PubMed: 2778432 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |