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微信小章| 产品名称： | pFEN207 |
|---|---|
| 商品货号： | TS168033 |
| Designations： | pFEN207 |
| GenBank Number： | M64618 |
| Species： | Chlamydia trachomatis |
| Depositors： | Rocky Mountain Laboratory, FE Nano, Rocky Mountain Laboratory |
| Applications： | produces protein 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase) |
| Vector： | Construct size (kb): 9.2 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC8 Intact vector size: 2.700 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII Construction: pBR322, Escherichia coli lacZalpha, M13mp8 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Chlamydia trachomatis Gene symbol: gseA Gene name: 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase) Contains complete coding sequence?: Y Strain: LGV-434 (serovar L2) Type of DNA: genomic Insert end: Sau3AI Insert end: Sau3AI Insert size (kb): 6.5 Cross references: DNA Seq. Acc.: M64618 Insert lengths(kb): 6.5 Gene product: 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase) gseA Target Gene: 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase) |
| Insert Size (kb)： | 6.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 2.8; EcoRI/HindIII--2.7, 1.35 (doublet), 0.84 (triplet), 0.64, 0.36, 0.27; ScaI/SacII--4.6, 2.4, 1.4, 0.96. Expresses the genus-specific lipopolysaccharide epitope of Chlamydia. The insert contains at least 2 open reading frames. The 1.4 kb ScaI/SacII fragment contains the complete coding sequence of gseA and has been sequenced. |
| Classification： | Chlamydiaceae, Chlamydia |
| References： | Nano FE, Caldwell HD. Recombinant clones of Chlamydia trachomatis lipopolysaccharide. US Patent 5,075,228 dated Dec 24 1991 Nano FE, Caldwell HD. Expression of the Chlamydial genus-specific lipopolysaccharide epitope in Escherichia coli. Science 228: 742-744, 1985. PubMed: 2581315 Belunis CJ, et al. A novel 3-deoxy-D-manno-octulosonic acid transferase from Chlamydia trachomatis required for expression of the genus-specfic epitope. J. Biol. Chem. 267: 18702-18707, 1992. PubMed: 1382060 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |