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微信小章| 产品名称： | Enterovirus A71 (EV-A71) |
|---|---|
| 商品货号： | TS168082 |
| Classification： | Enterovirus, Enterovirus A, Enterovirus A71 (EV-A71) |
| Agent： | Enterovirus A71 (EV-A71) |
| Strain： | BrCr |
| Applications： | Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Derived by passage of ATCC®xa0VR-784™ in Vero cells (ATCC® CCL-81™). This product replaces ATCC® VR-784™ and exhibits CPE when cells are infected.
Note: The sequences of ATCC® VR-784™ and ATCC® VR-1775™ have not been compared. |
| Effect on Host： | CPE in the form of cell rounding, sloughing and degeneration of the monolayer. |
| Recommended Host： | Vero (ATCC® CCL-81x99™) xa0xa0xa0 |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: xa0Plant cells 24-48 hours in advance and infect when cultures are 70-80% confluent.xa0 Remove medium, wash cell monolayer once with PBS, and inoculate with a small volume (eg. 1 mL per cm2) of virus diluted to provide a MOI of about 0.1.xa0 Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere.xa0 End adsorption by adding virus growth medium.xa0 Incubation: Incubate infected culture for 5-9 days at 37ºC in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture. |
| Name of Depositor： | NJ Schmidt |
| Chain of Custody： | Deposited as Enterovirus type 71 |
| Source： | Stool specimen from 2-month-old male with aseptic meningitis. |
| References： | Schmidt NJ, et al. An apparently new enterovirus isolated from patients with disease of the central nervous system. J. Infect. Dis. 129: 304-309, 1974. PubMed: 4361245 |