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微信小章| 产品名称： | 6G4.2.5 |
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| 商品货号： | TS168130 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma:lymphoblast B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain: BALB/c (B cell); BALB/c (myeloma) |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | An expression plasmid p6G425chim2 (ATCC 97055) was created to code for a chimeric murine-human Fab fragment of the monoclonal antibody. Cell lines producing the recombinant humanized antibody are available (ATCC CRL-12444 and ATCC CRL-12445) Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against rabbit interleukin 8 (IL-8) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against rabbit interleukin 8 (IL-8) |
| Comments： | An expression plasmid p6G425chim2 (ATCC 97055) was created to code for a chimeric murine-human Fab fragment of the monoclonal antibody. Cell lines producing the recombinant humanized antibody are available (ATCC CRL-12444 and ATCC CRL-12445) Animals were immunized with rabbit interleukin 8 (IL-8). Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells. The antibody blocks IL-8 binding to rabbit neutrophil receptors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10 exp6 cells/ml. Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Evey 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | IgG2a kappa , IgG2a |
| Name of Depositor： | Genentech, Inc. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Fong S, et al. Anti-IL-8 antibody fragments. US Patent 5,677,426 dated Oct 14 1997 Hsei V, et al. Methods of treating inflammatory diseases with anti-IL-8 antibody fragment-polymer conjugates. US Patent 6,468,532 dated Oct 22 2002 Hsei V, et al. Methods of treating inflammatory disease with anti-IL-8 antibody fragment-polymer conjugates. US Patent 6,458,355 dated Oct 1 2002 Gonzalez TN, et al. Humanized anti-IL-8 monoclonal antibodies. US Patent 6,133,426 dated Oct 17 2000 Gonzalez TN, et al. Humanized anti-IL-8 monoclonal antibodies. US Patent 6,117,980 dated Sep 12 2000 Gonzalez TN, et al. Nucleic acids encoding humanized anti-IL-8 monoclonal antibodies. US Patent 6,025,158 dated Feb 15 2000 |