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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS168196 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | EKA260 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Preceptrol®： | no |
| Genotype： | F- leu thi gal1 gal2 lac xyl ara hsdR deltatrpE5 lambdaDKC266(repA+) |
| Type Strain： | no |
| Comments： | derived from E. coli JMB9.1 Expresses the RepA protein required for replication of the P1 plasmids pVEX1211 and pVEX1212 (ATCC 77420, 77421). |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | DH Figurski |
| References： | Ayres EK, et al. Precise deletions in large bacterial genomes by vector-mediated excision (VEX). The trfA gene of promiscuous plasmid RK2 is essential for replication in several gram-negative hosts. J. Mol. Biol. 230: 174-185, 1993. PubMed: 8450534 |